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anti-ulbp4-pe (R&D Systems)

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R&D Systems anti-ulbp4-pe

a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and <t>ULBP4</t> on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.

Anti Ulbp4 Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-ulbp4-pe/product/R&D Systems
Average 90 stars, based on 1 article reviews

anti-ulbp4-pe - by Bioz Stars, 2026-04

90/100 stars

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1) Product Images from "γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects"

Article Title: γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

Journal: bioRxiv

doi: 10.1101/2021.10.14.464229

Figure Legend Snippet: a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and ULBP4 on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.

Techniques Used: Flow Cytometry, Staining, Expressing, Negative Control, Blocking Assay

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Journal: bioRxiv

Article Title: γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects

doi: 10.1101/2021.10.14.464229

Figure Lengend Snippet: a. Flow cytometry gating strategy on PDTO cells for analysis of surface staining. Selected cells were gated on single, live cells before quantification of staining signal. b. Histogram representation and count for surface staining of MHC-I, PD-L1, and β2m expression on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. Staining with isotype antibodies for each fluorochrome (PE, APC and FITC) were included as negative control. c. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining to test antitumor reactivity upon PDTO stimulation. Lymphocyte population was further gated on single cells, live and CD3+ cells, γδ TCR+ cells and CD8+ as well as CD8–CD4– cells. Reactivity of the sample was based on IFNγ+ cells of the selected population. d. Histogram representation and count for surface staining of NKG2D ligands MICA/B, ULBP1, ULBP2/5/6, ULBP3, and ULBP4 on two PDTO lines B2MWT and B2MKO after IFNγ pre-stimulation. e. Flow cytometry gating strategy on γδ T cell samples for analysis of intracellular staining after stimulation with PDTOs in the presence of NKG2D ligand blocking. Lymphocyte population was further gated on single cells, live and CD3+ cells, followed by γδ TCR+ and CD8+ as well as CD8– cells. Reactivity of final population was based on IFNγ+ or CD107a+ cells.

Article Snippet: Briefly, cells were incubated with human Fc receptor block (BioLegend) and stained with the different cell surface antibodies (1:10 anti-CD112-PE [clone R2.525, BD Biosciences], 1:10 anti-CD155-PE [clone 300907, R&D Systems], 1:50 anti-CD277/BTN3A1-PE [clone BT3.1, Miltenyi], 1:100 anti-HLA-A,B,C-FITC [clone W6/32, eBioscience], 1:20 anti-HLA-EBV421 [clone 3D12, BioLegend], 1:20 anti-HLA-G-APC [clone 87G, BioLegend], 1:300 anti-MICA/B-PE [clone 6D4, BioLegend], 1:10 anti-ULBP1-PE [clone 170818, R&D Systems], 1:20 anti-ULBP2/5/6-PE [clone 165903, R&D Systems], 1:20 anti-ULBP3-PE [clone 166510, R&D Systems], or 1:20 anti-ULBP4-PE [clone 709116, R&D Systems] for 45 min at 4°C.

Techniques: Flow Cytometry, Staining, Expressing, Negative Control, Blocking Assay

(A and B) Representative flow cytometric (A) histogram and (B) dot plots showing cell surface expression of NKG2D ligands compared with isotype control antibodies on primary human NK cells cultured in medium alone for 18 h. The numbers shown indicate the percentage of cells with staining above that of the isotype control. (C and D) Representative flow cytometric (C) histogram and (D) dot plots showing cell surface expression of NKG2D ligands compared with isotype control antibodies on primary human NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h. The numbers shown indicate the percentage of cells with staining above that of the isotype control. (E and F) Combined expression data of (E) ULBP2/5/6 and (F) ULBP4 on NK cells from 6 independent donors. **p < 0.01 in two-tailed Mann-Whitney test.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release 1

doi: 10.4049/jimmunol.1700647

Figure Lengend Snippet: (A and B) Representative flow cytometric (A) histogram and (B) dot plots showing cell surface expression of NKG2D ligands compared with isotype control antibodies on primary human NK cells cultured in medium alone for 18 h. The numbers shown indicate the percentage of cells with staining above that of the isotype control. (C and D) Representative flow cytometric (C) histogram and (D) dot plots showing cell surface expression of NKG2D ligands compared with isotype control antibodies on primary human NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h. The numbers shown indicate the percentage of cells with staining above that of the isotype control. (E and F) Combined expression data of (E) ULBP2/5/6 and (F) ULBP4 on NK cells from 6 independent donors. **p < 0.01 in two-tailed Mann-Whitney test.

Article Snippet: PE anti-MICA/B (159207), PE anti-ULBP1 (170818), PE anti-ULBP2/5/6 (165903), PE anti-ULBP3 (166510), PE anti-ULBP4 (709116), PE anti-TACE (FAB9301P), PE Mouse IgG 2A Isotype Control (20102), PE Mouse IgG 2B Isotype Control (133303), purified anti-NKG2D (149810) and Mouse IgG1 Isotype control (11711) were purchased from R&D Systems.

Techniques: Expressing, Cell Culture, Staining, Two Tailed Test, MANN-WHITNEY

(A, B and C) Percentage of human NK cells expressing ULBP2/5/6 cultured without cytokine or with IL-12, IL-15 and IL-18 alone or in combination for (A) 2 h, (B) 6 h, and (C) 18 h. (D, E and F) Percentage of human NK cells expressing ULBP4 cultured without cytokines or with IL-12, IL-15 and IL-18 either alone or in combination for (D) 2 h, (E) 6 h, and (F) 18 h. Combined data (mean +/− SEM) from 3 independent donors are shown in each panel.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release 1

doi: 10.4049/jimmunol.1700647

Figure Lengend Snippet: (A, B and C) Percentage of human NK cells expressing ULBP2/5/6 cultured without cytokine or with IL-12, IL-15 and IL-18 alone or in combination for (A) 2 h, (B) 6 h, and (C) 18 h. (D, E and F) Percentage of human NK cells expressing ULBP4 cultured without cytokines or with IL-12, IL-15 and IL-18 either alone or in combination for (D) 2 h, (E) 6 h, and (F) 18 h. Combined data (mean +/− SEM) from 3 independent donors are shown in each panel.

Techniques: Expressing, Cell Culture

(A and C) Representative flow cytometric plots of (A) ULBP2/5/6 and (C) ULBP4 staining on primary human NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h with or without NKG2D blockade. (B and D) Combined data from 8 independent experiments showing percentage (mean +/− SEM) of NK cells expressing ULBP2/5/6 and ULBP4 upon NKG2D blockade. *p< 0.05 in two-tailed Mann-Whitney test. ns, not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release 1

doi: 10.4049/jimmunol.1700647

Figure Lengend Snippet: (A and C) Representative flow cytometric plots of (A) ULBP2/5/6 and (C) ULBP4 staining on primary human NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h with or without NKG2D blockade. (B and D) Combined data from 8 independent experiments showing percentage (mean +/− SEM) of NK cells expressing ULBP2/5/6 and ULBP4 upon NKG2D blockade. *p< 0.05 in two-tailed Mann-Whitney test. ns, not significant.

Techniques: Staining, Expressing, Two Tailed Test, MANN-WHITNEY

(A–C) Relative expression of (A) ULBP2, (B) ULBP5 and (C) ULBP6 mRNA in IL-12, IL-15 and IL-18-stimulated human NK cells with or without NKG2D blockade. (D and F) Representative flow cytometric dot plots showing percentage of IL-12, IL-15 and IL-18-stimulated NK cells expressing (D) ULBP2/5/6 and (F) ULBP4 in the presence or absence of the TACE inhibitor TAPI-0. (E and G) Combined data from 5 independent experiments showing percentage of NK cells expressing ULBP2/5/6 and ULBP4 upon TACE inhibition. **p< 0.01 in two-tailed Mann-Whitney test. ns, not significant.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release 1

doi: 10.4049/jimmunol.1700647

Figure Lengend Snippet: (A–C) Relative expression of (A) ULBP2, (B) ULBP5 and (C) ULBP6 mRNA in IL-12, IL-15 and IL-18-stimulated human NK cells with or without NKG2D blockade. (D and F) Representative flow cytometric dot plots showing percentage of IL-12, IL-15 and IL-18-stimulated NK cells expressing (D) ULBP2/5/6 and (F) ULBP4 in the presence or absence of the TACE inhibitor TAPI-0. (E and G) Combined data from 5 independent experiments showing percentage of NK cells expressing ULBP2/5/6 and ULBP4 upon TACE inhibition. **p< 0.01 in two-tailed Mann-Whitney test. ns, not significant.

Techniques: Expressing, Inhibition, Two Tailed Test, MANN-WHITNEY

(A) TNF-α release in NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h in the presence or absence of the TACE inhibitor TAPI-0. These data are representative of 3 individual experiments. (B) TNF-α release from NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h with or without NKG2D blockade (mean +/− SD). These data are representative of at least 3 individual experiments. (C) Representative overlay histogram showing NKG2D expression in IL-12, IL-15, IL-18-activated human NK cells that had been transfected with four different NKG2D-specific siRNAs or negative control siRNA. The siRNAs shown are as follows: NKG2D siRNA#1 (solid line), NKG2D siRNA#2 (dotted line), NKG2D siRNA#3 (dashed line), NKG2D siRNA#4 (long dashes) and negative control siRNA (solid filled grey histograms). The cells were analyzed 24 hours after transfection. (D) TNF-α release (mean +/− SD) from human NK cells transfected with NKG2D-specific siRNAs or negative control siRNA. These data are representative of 2 individual experiments. (E) Representative overlay histogram showing ULBP4 expression in IL-12, IL-15, and IL-18-activated human NK cells that had been transfected with ULBP4-specific siRNA#1 (long dashes), ULBP4 siRNA#2 (solid line) or negative control siRNA (solid filled grey histogram). (F) TNF-α release from human NK cells transfected with ULBP4-specific siRNAs or negative control siRNA. These data are representative of 3 individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 in paired two-tailed Student’s t test. NC, negative control.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: NKG2D signaling between human NK cells enhances TACE-mediated TNF-α release 1

doi: 10.4049/jimmunol.1700647

Figure Lengend Snippet: (A) TNF-α release in NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h in the presence or absence of the TACE inhibitor TAPI-0. These data are representative of 3 individual experiments. (B) TNF-α release from NK cells stimulated with IL-12, IL-15 and IL-18 for 18 h with or without NKG2D blockade (mean +/− SD). These data are representative of at least 3 individual experiments. (C) Representative overlay histogram showing NKG2D expression in IL-12, IL-15, IL-18-activated human NK cells that had been transfected with four different NKG2D-specific siRNAs or negative control siRNA. The siRNAs shown are as follows: NKG2D siRNA#1 (solid line), NKG2D siRNA#2 (dotted line), NKG2D siRNA#3 (dashed line), NKG2D siRNA#4 (long dashes) and negative control siRNA (solid filled grey histograms). The cells were analyzed 24 hours after transfection. (D) TNF-α release (mean +/− SD) from human NK cells transfected with NKG2D-specific siRNAs or negative control siRNA. These data are representative of 2 individual experiments. (E) Representative overlay histogram showing ULBP4 expression in IL-12, IL-15, and IL-18-activated human NK cells that had been transfected with ULBP4-specific siRNA#1 (long dashes), ULBP4 siRNA#2 (solid line) or negative control siRNA (solid filled grey histogram). (F) TNF-α release from human NK cells transfected with ULBP4-specific siRNAs or negative control siRNA. These data are representative of 3 individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 in paired two-tailed Student’s t test. NC, negative control.

Techniques: Expressing, Transfection, Negative Control, Two Tailed Test

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1) Product Images from "γδ T cells are effectors of immune checkpoint blockade in mismatch repair-deficient colon cancers with antigen presentation defects"

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